148 Disruption of nucleolar functions variably affect epidermal differentiation
نویسندگان
چکیده
Nucleoli are multifunctional organelles and the site of ribosome synthesis, which is critical for protein translation. A hallmark epidermal differentiation expression a defined set proteins in suprabasal keratinocytes where each layer has unique identity profile. However, link between nucleolar function cell remains unexplored. To determine whether nucleoli play role process differentiation, we examined number area different layers mature 3D human skin equivalents (3D HSE). As became more differentiated, (fibrillarin punctae) (relative to nucleus) decreased (P ≤ 0.1). Blocking by silencing EPHA2 receptor tyrosine kinase reduced basal increased their < 0.0001). These results demonstrate dynamics during keratinocyte differentiation. address structure and/or synthesis important knocked down POLR1A (segregating structure) or UTP4 (disrupting processing rRNA without segregating nucleoli) HSE. Knockdown either reduces synthesis. Assessment tissue morphology revealed both knockdowns stratification. there were contrasting effects on extent knockdown accelerated with deposition keratohyalin granules enhanced cornification. In contrast, loss impaired these aspects terminal Further, filaggrin was siPOLR1A HSE sections, but samples. The changes characteristics variable segregation stratification suggest functional Future studies will tease out mechanisms
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2021
ISSN: ['1523-1747', '0022-202X']
DOI: https://doi.org/10.1016/j.jid.2021.02.167